| abstract |
The invention relates to the technical field of organoid culture, and specifically discloses a cervical squamous cell carcinoma organoid culture medium and a construction method thereof. Cervical squamous cell carcinoma organoid culture medium, comprising the following components: basal medium, HEPES buffer pair, penicillin-streptomycin solution, nutritional supplements and specific factors; the specific factors include nicotinamide, N-acetylcysteine , Y‑27632, Noggin Factor, A83‑01, EGF, FGF10, Forskolin and Hydrocortisone. The invention develops a cervical squamous cell carcinoma organoid culture medium for cervical squamous cell carcinoma, improves the culture success rate and long-term maintenance rate of the cervical carcinoma organoid, and the cultured cervical squamous cell carcinoma organoid can maintain the histological morphology and genetics of the original tumor tissue. The developed cervical squamous cell carcinoma organoid medium is beneficial to provide new ideas for cervical cancer research. |