| abstract |
The invention discloses an African swine fever virus antigen recombinant tandem epitope protein, which is formed by connecting the African swine fever virus CD2v protein and the B cell epitope of the MGF360-505R protein in series with flexible peptides. The inclusion body has a higher content, has a certain solubility, is reactive with serum antibodies, and can be used for the detection of antibody ELISA. Also disclosed are the construction method of the African swine fever virus antigen recombinant tandem epitope protein, the application in antibody ELISA detection and the antibody ELISA detection kit. The application and antibody ELISA detection kit of the present invention has good specificity, good repeatability and stability, and high sensitivity, can accurately identify serum samples generated after immunization of ASFV wild-type and CD2v gene-deleted strains, and has certain properties for ASF detection. The reference value of serological assays based on conserved epitopes is expected to provide a wider diagnostic range. |