| abstract |
The invention relates to a method for preparing Aspergillus flavus uric acid oxidase by intracellular expression of yeast, comprising: after inducing the intracellular expression of Aspergillus flavus uric acid oxidase in recombinant yeast, placing thalline in a lysis buffer to disrupt cells; wherein the The cytolysis buffer comprises an additive selected from one or a combination of dithiothreitol, tris(2-carboxyethyl)phosphine, cysteine, and glutathione. The present invention inhibits the degradation of yeast intracellular protein by optimizing the homogenization buffer to ensure the integrity of the target protein; and can also improve cell breaking conditions such as reducing homogenization pressure, thereby reducing production energy consumption and reducing equipment requirements. |