| abstract |
The invention belongs to the technical field of chemical analysis, and specifically relates to a method for identifying 6 components in Shujin Huoxue Pills by UPLC-Q-TOF method. Piedrin, amygdalin, catechin and ± protohematoxylin B. The method adopts Waters ultra-high-speed liquid chromatograph and special chromatographic column for ultra-high-speed liquid chromatograph with small particle size to carry out gradient elution, and mobile phase A is a mixed solution of ammonium formate and formic acid, wherein the concentration of ammonium formate is 0.02 mmol/L, The volume concentration of formic acid was 0.1%, and the mobile phase B was a mixed solution of formic acid and acetonitrile, of which the volume concentration of formic acid was 0.1%; the detection instrument was quadrupole time-of-flight tandem mass spectrometry, and the scanning mode was electrospray ionization negative ion mode. The method established by the present invention can accurately identify 6 components in Shujin Huoxue Pill, and judge whether the medicine contains Ticrup, Achyranthes, Rhizoma Drynariae, Peach Kernel, Catechin, Hematoxylin, and comprehensive evaluation. The quality of Shujin Huoxue Pills is of great significance. |