| abstract |
The invention belongs to the technical field of pathogen detection, and discloses a method for rapid visual detection of SADS-CoV based on CRISPR-Cas12a system, comprising the following steps: using SADS-CoV cDNA, PEDV cDNA, TGEV cDNA, PDCoV cDNA and H 2 O as templates, using The RPA reaction primer pair was used for the RPA reaction, and the specific guide RNA, crRNA and fluorescent quenching reporter probe were used to carry out the Cas12a visual digestion reaction of the RPA reaction product. The invention is designed for the relatively conserved region on the SADS-CoV N gene sequence, has good specificity, can accurately detect SADS-CoV from a series of coronaviruses related to porcine diarrhea, has high sensitivity and can reach a single copy level, has a high detection rate and Can be visualized. |