| abstract |
The invention discloses a gene encoding a recombinant UNG enzyme, the base sequence of which is shown in SEQ ID NO: 2, and the amino acid sequence of the recombinant UNG enzyme is shown in SEQ ID NO: 1. The encoded UNG enzyme has strong activity, the UNG enzyme encoding gene can be efficiently and soluble expressed in Escherichia coli, and a high-purity recombinant UNG enzyme can be obtained through a two-step purification process. |