| abstract |
Provided are recombinant polypeptides comprising a protein of interest, a protein localization tag, and a protease cleavage site between the protein of interest and the protein localization tag. In certain embodiments, the recombinant polypeptide further comprises a protease, wherein the protease cleavage site is the cleavage site of the protease. Nucleic acids encoding the recombinant polypeptides, cells comprising such nucleic acids, and compositions (eg, pharmaceutical compositions) comprising such cells are also provided. Also provided are methods of modulating the cellular localization of a protein of interest and methods of administering regulatable cell-based therapy to an individual in need thereof. |