http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114740192-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5e46b2382ec685f7cf17c2dc10057e7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54346 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2022-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd6a728894244190302a80c71f71a5ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49e509afff24a0781e05509f574c5649 |
| publicationDate | 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114740192-A |
| titleOfInvention | A kind of labeling method of high concentration colloidal gold |
| abstract | The invention discloses a method for labeling high-concentration colloidal gold. The high-concentration colloidal gold solution is diluted with buffer solutions of different pH respectively to form colloidal gold solutions of different pH, and proteins to be labeled are respectively added to each colloidal gold solution. , and then determine the optimum pH for labeling by high-salt damage test. Dilute the high-concentration colloidal gold solution with the labeling buffer with the optimum labeling pH, then divide it into several equal parts, and add the protein solution to be labeled respectively to form a mixed solution of different protein final concentrations. appropriate amount of protein labeling. According to the conditions for determining the optimal labeling and pH optimal protein labeling amount, the labeling mixture is prepared, and then a blocking reaction is performed to obtain a colloidal gold-labeled protein complex. The gold particles do not aggregate in the whole labeling process of the invention, the volume of the reaction system is small, 60% of labor is saved, and protein materials are saved. |
| priorityDate | 2022-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.