| abstract |
The invention discloses a SERS probe biosensor and a preparation method and use method thereof, and relates to the field of biotechnology. The SERS probe biosensor includes a DNA probe 1, a DNA probe 2 and an aptamer, and the DNA probe 1 and the DNA probe 2 have at least 5 complementary base pairs, and the DNA probe 1 and the DNA probe The remaining bases on 2 are complementary to the bases on the aptamer, and DNA probe 1, DNA probe 2 and the aptamer are paired to form a Y shape, and the DNA probe 1 is also covalently linked Raman reporter molecule-modified gold nanoparticles, and the DNA probe 2 is covalently attached to a surface-modified streptavidin magnetic bead. The SERS probe biosensor prepared by the invention has the advantages of high sensitivity, high specificity, low sample consumption and high accuracy, and can effectively realize accurate diagnosis of acute kidney injury. |