| abstract |
The invention discloses a method for synthesizing Tirzepatide, comprising the following steps: step 1, amino acids at positions 7-8, amino acids at positions 10-11, and amino acids at positions 32-33 of the Tirzepatide sequence are used as pseudo-pro dipeptides; The amino acid at position 30 is used as a dipeptide; step 2, use a condensing agent to solid-phase synthesize amino acids at other positions of the tirzepatide sequence in sequence in the reaction solvent, and use Fmoc removal reagent to synthesize fully protected Tirzepatide; step 3, use a cleavage reagent for cleavage Precipitation to obtain Tirzepatide; the method of the invention improves the purity of the crude peptide, greatly reduces the material cost and purification cost, and is conducive to industrial scale-up production. |