http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114729352-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66432c0b0cb751e6e25318ccc2dc13a7 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D277-64 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2020-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63f5c98c42db216b7a73a8ffa6c24699 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5157591f36f442fee7fcda8410080ee0 |
publicationDate | 2022-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114729352-A |
titleOfInvention | Fluorescein derivatives and synthetic methods thereof |
abstract | The present disclosure relates to fluorescein derivatives and synthetic methods of fluorescein derivatives. The method for synthesizing fluorescein derivatives involves performing a first reaction by using a toxic phenol derivative as a substrate, and by using a thermostable dehalogenase, a group of free radical scavenging enzymes, a group of polyphenol oxidases, and a FADH 2 generating system. Toxic phenol derivatives are reacted in buffer solution to obtain benzoquinone. The FADH 2 generation system is capable of generating FADH 2 , which is a substrate for thermostable dehalogenases. Furthermore, a second reaction was performed between the derivatized benzoquinone and D-cysteine to obtain a fluorescein derivative capable of emitting light at a wavelength of 600-700 nm. Therefore, fluorescein derivatives can be used in various fields such as medical research, drug research and other detection techniques. |
priorityDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 669.