| abstract |
The invention discloses a S-adenosyl-L-methionine δ24-sterol-C-methyltransferase mutant C24MTgm-M11. Strain MG1655 (ΔptsGΔfumACΔfumB,panD,aspA,C24MTgm) was constructed from the encoding polynucleotides of the enzyme mutants. Strain MG1655 (ΔptsGΔfumACΔfumB,panD,aspA,C24MTgm-M11) could produce 480 mg/L of 3-amino in shake flask fermentation isobutyric acid. Compared with the wild-type strain C24MTgm, the strain containing the mutant C24MTgm-M11 significantly increased the production of 3-aminobutyric acid by 5.8 times. |