| abstract |
The invention belongs to the technical field of immunodetection, and discloses a preparation method of Salmonella Enteritidis colloidal gold immunochromatographic test strip, comprising: using an optimized Tris-base auxiliary method to prepare colloidal gold, and coupling with Salmonella Enteritidis monoclonal antibody to obtain Immunolabel; assemble the screened sample pad, the binding pad immobilized with the immunolabel, the NC membrane immobilized with the T/C line antibody, the absorbent paper and PVC to obtain the Salmonella Enteritidis colloidal gold immunochromatographic test strip. The present invention prepares colloidal gold with better uniformity and stability based on the Tris-assisted method, and the Salmonella Enteritidis colloidal gold immunochromatographic test strip established with this as a marker has high sensitivity, good specificity, good repeatability, and various aspects. The performance meets the requirements of rapid clinical detection, which proves that the Salmonella Enteritidis colloidal gold immunochromatographic test strip prepared based on the Tris-assisted method can be used for clinical field detection. |