abstract |
The invention discloses a method for detecting Helicobacter pylori by fluorescence polarization, which is based on gold nanoparticles and Helicobacter pylori aptamer nanospheres, and mainly includes the following steps: S1: preparation of gold nanoparticles; S2: assembly of nanospheres; S3: detection of Helicobacter pylori; S4: quantitative detection of actual samples containing Helicobacter pylori. The invention has excellent detection effect of Helicobacter pylori, the dynamic response range is from 0.9 CFU/mL to 0.9×10 7 CFU/mL, the operation is convenient, and the detection is rapid. At the same time, the invention can be extended to identify other bacteria by changing the aptamer. Achieve rapid detection and differentiation of bacteria at low cost. |