http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114705856-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_52e44eef04113be38b5c904930e36687 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6525a1be15dbc026ff08b766202b49b5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-165 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ecd188d01fafccb103310929356c5e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c77e99e03c80043bc9d7226ae989ee6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c83961b461f28c105fb4b64b0e0d7af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b694a1c4be1b7f728b6324554de35d95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_189355701061d109e14381abff20dcfb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf667ad5e8ce0bcc587acb8fc97adc7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4aa14b856d8306052c25265ad9eccd7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24e442c9b1ecb2d856eb9ec47283e8ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96220fb0949434bdb175bffc96f7ff3a |
| publicationDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114705856-A |
| titleOfInvention | Method, reagent and kit for detection of antigen content of delta variant strain in novel coronavirus vaccine |
| abstract | The embodiment of the present invention discloses a method for detecting the antigen content of a delta variant strain in a novel coronavirus vaccine, comprising: preparing a goat polyclonal antibody against the S protein receptor binding region (RBD) epitope of the novel coronavirus delta variant strain Coat the enzyme-labeled plate, seal the enzyme-labeled plate, wash the plate, and pat it dry; add the vaccine antigen sample to be tested and the antigen standard substance respectively, and dilute them in different gradients in the plate, and then add the antigen sample of the vaccine to be tested and the antigen standard. The ELISA plate of the antigen standard was sealed and incubated at 37°C for 1 h to 2 h, washed and patted dry; a rabbit polyclonal antibody against the epitope of the S protein receptor binding region of the new coronavirus delta variant was added as the primary antibody, and the The enzyme-labeled plate containing the primary antibody was sealed and incubated at 37°C for 1 h to 2 h, washed and patted dry; goat anti-rabbit Ig antibody labeled with horseradish peroxidase was added as the secondary antibody at an appropriate dilution to obtain a secondary antibody containing the primary antibody. After the plate was sealed and incubated at 37°C for 1 h to 2 h, the plate was washed; after adding a chromogenic solution for color reaction, the antigen content of the vaccine antigen sample to be tested was determined. The invention can significantly increase the antigen content of the delta variant strain in the new crown vaccine. |
| priorityDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.