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publicationDate 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114703302-A
titleOfInvention Method for detecting Mycoplasma gallis synovium and reagents used therefor
abstract The invention discloses a method for detecting Mycoplasma gallis synovium and a reagent used therefor, belonging to the technical field of nucleic acid or microorganism determination or inspection methods. The invention discloses a method for detecting or assisting in the detection of Mycoplasma gallis synovium. The method comprises using a reagent or a kit to perform digital PCR on a sample to be tested, and determining whether the sample to be tested is Mycoplasma gallis synovium or whether the sample to be tested is determined according to the digital PCR product. Contains Mycoplasma gallis synovium or, whether it is infected with Mycoplasma gallis synovium, the reagent or test kit includes a primer probe of Mycoplasma gallis synovium, and the primer probe of Mycoplasma gallis synovium contains nucleotides by specific amplification The sequence is composed of primers for the genomic DNA fragment of Mycoplasma gallis synovium including the DNA fragment of SEQ ID No. 1 and a probe that specifically binds to the DNA fragment of the nucleotide sequence of SEQ ID No. 1. The method can be used to detect environmental samples and food.
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