http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703267-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a14601f6ed98b86d479ca76ceeaab43 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2022-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbab81c764b49df3b6451a086b7fd9e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4c00610a6d0cc31a7341ad26e49dd3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbf3675a18a060bcc48cc61cfec7b461 |
| publicationDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114703267-A |
| titleOfInvention | A Sanger sequencing method based on tailed primer design |
| abstract | The invention relates to the technical field of gene sequencing, in particular to a Sanger sequencing method based on the design of tailed primers, comprising the following steps: (1) selecting a specific primer sequence according to sample DNA, and then adding a specific primer sequence to the 5' end of the specific primer sequence. The general primer sequence is used to obtain the tailed primer sequence, and the tailed primer is synthesized according to the tailed primer sequence; (2) the PCR amplification reaction solution is prepared by using the tailed primer, the sample DNA is added, and the PCR amplification procedure is performed to obtain the PCR amplification product; (3) Purify PCR amplification products; (4) Select universal primers as sequencing primers, and perform cycle sequencing reaction on the purified PCR amplification products; (5) After purification, denaturation, and on-machine sequencing of cycle sequencing reaction products, sample DNA is obtained The sequence of the present invention can not only save the workload of Sanger sequencing, but also reduce the possibility of primer error addition through the combination of universal sequence and specific sequence primers. |
| priorityDate | 2022-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.