http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703136-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-32 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate | 2022-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114703136-B |
| titleOfInvention | A method for isolation and primary culture of newborn piglet intestinal neurons |
| abstract | The invention belongs to the field of biotechnology, and discloses a method for separating and primary culturing intestinal neurons of newborn piglets. Digest the intestine with the digestion medium, and collect the cell pellet; Step 3: Add trypsin to the cell pellet obtained in Step 2 to digest the intestine, and collect the cell pellet; Step 4: Resuspend the cells in the cell culture medium and resuspend the cells obtained in Step 3 , and inoculated onto porous cell culture slides coated with laminin and Poly-D lysine; the cell culture medium was changed regularly, and the cells were collected and tested after 5 days for cell protein staining. This method can successfully extract enteric neurons from piglet intestines and culture them in primary culture. |
| priorityDate | 2022-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.