http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703047-A

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filingDate 2022-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39bf405e27f263847ce080533d7051e0
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publicationDate 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114703047-A
titleOfInvention A multipurpose slide device for animal cell disassembly and combined culture
abstract A multipurpose slide device for dismantling, combining and culturing animal cells, comprising: a glass slide frame and a culture snap ring chamber, a self-locking fixing frame and a flat-head micro-sampler head top cover, which utilizes cells to culture on the slide It grows adherently and provides nutrients for cell proliferation through the nutrients in the culture snap-ring chamber (feeder pool) medium. Weld-lock stainless steel flat-headed injection needle is fixed by titanium alloy conical cover, the needle seat has an anti-contamination protection cap, and the titanium alloy conical cover has an air filter membrane installation position based on short-tail stainless steel flat-headed needle seat in the middle, which is used for aeration culture. gas exchange. Fluorescent staining and observation can be performed during the cell culture process, or the slides can be stained by directly adding fluorescent dyes in the culture collar chamber after replacing and washing the medium. It reduces the impact on cell structure and morphology due to the separation of cells and the preparation process, and solves the difficult problem of structural microscopic observation and live cell fluorescence observation during long-term culture.
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Total number of triples: 25.