| abstract |
The present invention provides a method for constructing STAT1 knockout mice (referred to as 4hEF Tg STAT1 ‑/‑ mice) by transgenic CD81, CLDN1, OCLN and SRB1 four receptors combined with CRISPR/Cas9 technology. The four-receptor transgenic mice were obtained by injection, and the target gene sgRNA was designed based on the CRISPER/Cas9 system to obtain STAT1 knockout mice by microinjection; the four-receptor transgenic mice were crossed with STAT1 knockout mice to obtain heterozygous F1 generation , and then obtain homozygous 4hEF Tg STAT1 ‑/‑ mice through F1 generation selfing, namely the mice of the present invention, which can be used to establish a chronic HCV infection mouse model. |