http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114672592-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b79b6be48b8f6ef4d29116f58201c476 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-707 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2022-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bd75a7b6dacb98b92474acab2521a5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b699e0a9bb168153c95c5b700e62b613 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de4a1ef6c7b13e37bb136df89c0a7205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4fbe53a9bc42f8fa256a2f69a3f9c09 |
| publicationDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114672592-A |
| titleOfInvention | A specific primer, probe and kit for real-time fluorescent detection of multiplexed viruses in xenograft donor pigs |
| abstract | The invention discloses a specific primer, probe and kit for real-time fluorescent detection of multiplexed viruses in xenotransplantation donors. The nucleic acid sequences of porcine hepatitis E virus (PHEV) were compared and analyzed, and the 1332-1430 segment of the PERV-GAG gene (all three types of PERV-ABC were highly conserved), the SI-HA gene, and the PHEV-ORF3 gene were used as amplification targets. Site, select the segment without secondary structure and highly conserved, according to the basic principles of primer probe design, using primer analysis software and manual judgment, and after many experiments optimization, it is determined that it is suitable for real-time fluorescence quantitative Real Time-PCR (RT -PCR or qPCR) multiple pairs of primers and probes. The invention also discloses a corresponding kit and method for rapid screening and quantitative detection. The present invention provides methods for simultaneous screening of donor porcine endogenous retrovirus (PERV), swine influenza virus (SI) and porcine hepatitis E virus (PHEV). |
| priorityDate | 2022-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.