http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114672470-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b9fecddfb2711265ada549d1cd13a132 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
filingDate | 2022-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70ea81a8108353da319817b5717af0d3 |
publicationDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114672470-A |
titleOfInvention | A cytochrome P450 BM3 mutant enzyme and its application in biocatalytic synthesis of p-hydroxybiphenyl |
abstract | The invention provides a cytochrome P450BM3 mutant enzyme and its application in biocatalytic synthesis of p-hydroxybiphenyl, wherein the cytochrome P450BM3 mutant enzyme is at the 87th position of the amino acid sequence of wild-type cytochrome P450BM3 monooxygenase and amino acid mutation at position 267, specifically including: A. The cytochrome P450BM3 mutant enzyme is a single-site point mutation, and the point mutation includes: E267V or F87G; B, or the cytochrome P450BM3 mutant enzyme Mutations at two sites are E267V and F87G. The cytochrome P450 monooxygenase mutant P450BM3 provided by the invention has a nearly 1.5-fold increase in the hydroxylation activity of biphenyl compared with the wild-type CYP activity, and has a relatively stable catalytic effect. And the reaction conditions are mild, no high temperature and high pressure environment. |
priorityDate | 2022-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 299.