| abstract |
The invention belongs to the field of molecular biology, and specifically discloses a recombinant leukocyte inhibitory factor and a leech peptide chimeric protein mutant. Based on TNHH, the present invention mutates the cysteine that easily leads to mispairing in translation and expression, and mutates some non-polar amino acids at the thrombin binding site to other amino acids to obtain TNHH mutants. TNHH is a bifunctional chimeric protein. We solved two problems at the same time through gene site-directed mutagenesis, which not only reduced its isoform content, improved protein purity, but also greatly improved its biological activity. high practical significance. |