| abstract |
The invention discloses a method for constructing a zebrafish obesity model, comprising: placing adult zebrafish males and females in a mating box, collecting eggs after mating, culturing and incubating healthy eggs at a constant temperature, and selecting healthy zebrafish juveniles after fertilization; BPA A mixed aqueous solution was prepared with DEHP and acetone for feeding the zebrafish larvae; cooked egg yolk was fed after hatching, and the zebrafish obesity model was established on the 21st day after fertilization. The method of the zebra obesity model constructed by the invention has the advantages of short time, good economy and high efficiency, and can provide a convenient, fast and efficient research tool for the toxicity assessment and mechanism research of environmental endocrine disruptors, as well as the screening of therapeutic drugs. |