| abstract |
The present invention provides a kit and method for detecting circulating PD-L1 positive cells, the kit includes a detection reagent and a PD-L1 positive cell capture reagent; the detection reagent includes PD labeled with a first non-human marker ‑L1 antibody, leukocyte common antigen antibody labeled with the first fluorescent molecule, cancer cell antigen antibody labeled with the second fluorescent molecule, anti-nth non-human marker antibody labeled with the third fluorescent molecule, … Anti-n-2 non-human marker antibody labeled with human marker, and anti-n-1 non-human marker antibody labeled with n-th non-human marker; wherein, n is a positive integer greater than or equal to 1, The first, second, and third fluorescent molecules have different emission wavelengths, and the first-nth non-human marker is a different non-human marker, and the kit is used to detect circulating PD-L1 positivity Cells have higher sensitivity and lower detection limits. |