| abstract |
The present invention relates to a Fab-albumin-binding peptide fusion protein and a preparation method and application thereof. The Fab-albumin-binding peptide fusion protein comprises a Fab fragment that binds to TNF-α, an albumin-binding peptide (ABD) and a connecting peptide segment. wherein the albumin-binding peptide is linked to the C-terminus of the Fab fragment through a linker peptide. The Fab-albumin-binding peptide fusion protein not only retains the antigen-specific binding ability of the Fab fragment, but also has the ability to bind to albumin, and can specifically bind to plasma albumin in vivo to significantly prolong its own half-life. The Fab-albumin-binding peptide fusion protein can be efficiently expressed in prokaryotic cells, and the purification process is simple and efficient. |