http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114617062-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2022-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114617062-B
titleOfInvention Tissue culture and fast propagation of crocodile flower
abstract The invention discloses a method for fast propagation of crocodile flower by tissue culture, and belongs to the technical field of plant tissue culture. The method includes the following steps: (1) selection and disinfection of explants: taking new shoots as explants for disinfection; (2) inducing growth of axillary buds: cutting the disinfected shoots into stem segments and inserting into the induction medium (3) Induction and subculture of cluster buds: the side shoots obtained by the growth of axillary buds are cut into small pieces and inserted into the differentiation medium, and the cluster buds are induced to grow, and then the obtained cluster buds are inserted into the differentiation medium for sub-generation Cultivate; (4) rooting culture: the clustered shoots obtained by subculture are cut off and inserted into the rooting medium to cultivate to obtain rooting seedlings; (5) hardening and transplanting: after the gained rooting seedlings are hardened for 3d, transplanted to a sand bed nourish. The invention establishes a fast, stable and effective rapid propagation technology system of crocodile flower, can be effectively used in production, and provides scientific basis and technical guarantee for the development and utilization of large-scale production of crocodile flower in the future.
priorityDate 2022-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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