| abstract |
A cell culture method comprising (i) culturing cells in a cell culture medium, and (ii) placing cells selected from the group consisting of aconitic acid (AA), leucic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid At least one metabolite of acid (MSA), trigonelline (TRI) and N-acetyl putrescine (NAP) is maintained below the inhibitory concentration of the at least one metabolite in the cell culture medium. |