http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114609381-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab6a707674d3df43ed1b013d9fc9912d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-37 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54346 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a097d739d9e6a10e763bf3fea16256f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a86264b775ec7499dfb5e08225492282 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b88daa994a1b115e3dfc315adddb85a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55fd558b9aea91f4cee9453bb1c2899b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135b9408da0bd16f68cb92ac374d0586 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eab5b96fc3c3bf6d3026888b0cfc0804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ee3664f700bc97d8da67fc0fada2f09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_482613a3f837b06f9a237b386dd5096e |
| publicationDate | 2022-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114609381-A |
| titleOfInvention | A method for detecting TeA content based on magnetic separation and gold enzyme immune complex |
| abstract | The invention discloses a method for detecting TeA content based on magnetic separation and gold enzyme immune complex, and constructs a gold particle-antibody/HRP nano complex based on the simultaneous loading of horseradish peroxidase (HRP) and anti-TeA antibody on the surface of gold nanometers, That is, gold enzyme immune complex; TeA-coated original (hapten-OVA) was immobilized on the surface of carboxylated magnetic nanobeads by EDC/NHS method, and functionalized magnetic nanoparticle probes were constructed and coated in enzyme-labeled wells. The hapten-OVA directly competes with the TeA in the sample to bind to the anti-TeA antibody on the surface of the gold-enzyme immune complex; the substrate color reaction is used to detect the change in absorbance to quantitatively analyze the TeA content in the sample. By comprehensively utilizing the advantages of nanomagnetic beads for rapid separation and enrichment of target compounds, the large specific surface area of gold nanoparticles to increase HRP loading, and the double catalytic sensitization effect of the enzyme-like activity of gold nanoparticles on substrates, the separation of magnetic beads and gold enzymes were established. Immune complex signal sensitization detection method for rapid detection of TeA content. |
| priorityDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.