| abstract |
The invention discloses a method for detecting hydrogen peroxide by a high-sensitivity colorimetric/electrochemical dual-mode sensor based on Fe 3 O 4 @MoS 2 -Au NPs with high peroxidase activity, comprising: constructing a magnetic composite material Fe 3 O 4 @MoS 2 ‑Au NPs; the magnetic biocomposite, TMB and different concentrations of H 2 O 2 were incubated for reaction, the magnetic composite was separated, sulfuric acid was added, the UV absorption curve of the sample was measured, and the standard curve was established with the maximum UV absorption value; Obtain the maximum UV absorption value of the sample to be tested, and substitute it into the standard curve to obtain the H 2 O 2 concentration. It also includes: reacting the magnetic biocomposite material with H 2 O 2 , inserting an electrode, measuring the electrochemical curve of the sample at ‑0.7V～‑0.1V, and establishing a standard curve with the maximum signal value; measuring the maximum signal value of the sample to be tested, Substitute into the standard curve to obtain the H 2 O 2 concentration in the sample to be tested. The invention can detect H 2 O 2 simply, quickly and sensitively. |