| abstract |
The invention provides an in vitro transfection and transformation device for eukaryotic and prokaryotic cells by ultrasonic perforation, comprising: a perforated plate provided with a plurality of accommodating holes; a driving mechanism; a water tank for holding deionized water; ; heating element; at least two ultrasonic transducers of different frequencies, which are arranged below the orifice plate and whose emitting end is immersed in deionized water; the driving mechanism drives the orifice plate to displace in the horizontal plane, so that the receiving hole to be measured is located in the corresponding ultrasonic wave in the far-field region directly above the transducer. The accommodating hole is located in the far-field area of the sound field of the ultrasonic transducer. During transfection, the sound intensity of the ultrasonic transducer sound field changes little with the spatial position, and the spatial characteristics of the sound field are stable, which is beneficial to ensure relatively stable ultrasonic cavitation intensity and ultrasonic wave. The perforation effect and the repairable ultrasonic perforation of the cell membrane make the cell transfection efficiency high and the cell survival rate high. The transmitting end of the ultrasonic transducer is placed in deionized water, which can avoid the risk of contamination caused by direct contact with the cell suspension. |