http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114591930-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114591930-B |
| titleOfInvention | Enhanced reverse transcriptase, coding DNA, kit and its application in RNA library construction |
| abstract | The invention provides an enhanced reverse transcriptase, which is characterized in that: on the basis of the wild-type reverse transcriptase mmlv, it has nine amino acid sites of L22V; D145Q; C240G; T288E; L319A; L385K; T431Q; H595I; Q563A Mutation, the amino acid sequence of which is shown in SEQ ID NO:1. And disclosed its coding DNA, kit and its application in RNA library construction. Compared with the conventional RNA library building kit, the rapid RNA library building kit containing the enhanced reverse transcriptase of the present invention saves the second-strand cDNA synthesis step, shortens the library building time, and significantly reduces the number of cycles of library amplification , shortening the construction time, 500pg RNA library construction can be completed in 3.5h. |
| priorityDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 374.