http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114591886-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e2efaa482535230172df5e863f36c00 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate | 2022-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3533767506d701348d3977343180441e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3056f113a2b09e2d1b0e8172a83e0946 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9939b1c793280d712cbcc874df464863 |
| publicationDate | 2022-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114591886-A |
| titleOfInvention | A high-purity extraction method of yellow catfish liver mitochondria-endoplasmic reticulum coupling |
| abstract | The invention relates to the technical field of mitochondrial extraction, and discloses a high-purity method for extracting mitochondrial endoplasmic reticulum coupling from the liver of yellow catfish. Use BSA-containing buffer to wash oil; trypsin digestion according to liver content; digested tissue is slowly homogenized, homogenate is sonicated after adding protease inhibitors; the homogenate is centrifuged at low speed to remove unbroken cells . The high-purity yellow catfish liver mitochondria-endoplasmic reticulum coupling extraction method has the advantages of simple operation, high extraction efficiency, less MAM impurities extracted from tissue, and trypsin to digest inter-tissue impurities, reducing pollution and improving cell extraction efficiency. , and the cells were disrupted by suitable ultrasonic frequency to increase the extraction efficiency of MAM. In this way, the purpose of extracting the mitochondria-endoplasmic reticulum coupling structure of yellow catfish was achieved. |
| priorityDate | 2022-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 101.