http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114578049-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_31b7ffe2614fed802e31da4c85a029f8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2022-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88d6b4f5a99b79f5737436a1eb930483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_204d2e00e66b3d0e7e5cbd1795fabc3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6a875e2333ea72184e603d78a220f48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_004ee02c06354f54374d8825ca497ec1 |
| publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114578049-A |
| titleOfInvention | Preparation method and application of a Western blot detection kit |
| abstract | The invention provides a preparation method and application of a western blotting detection kit, which at least comprises: a nitrocellulose membrane embedded with a recombinant antigen and an alkaline phosphatase-labeled mouse anti-pig IgG. The kit preparation method includes: electrophoresis of the recombinant antigen in a polyacrylamide gel under the conditions of a voltage of 100V and a current of 1-2A, and the electrification time is 45min; the product obtained in step 1 is subjected to membrane transfer treatment with a membrane transfer solution; The membrane after transfer membrane treatment was immersed in the blocking solution for blocking treatment; then the membrane was air-dried to obtain a nitrocellulose membrane embedded with recombinant antigens; the activated alkaline phosphatase was used for the detection of mouse anti-pig IgG by sodium periodate method. After labeling, the unbound alkaline phosphatase was removed by dialysis to obtain alkaline phosphatase-labeled mouse anti-pig IgG, and then the alkaline phosphatase-labeled mouse anti-pig IgG was diluted with enzyme-labeled diluent containing 0.12‰ green pigment, And together with the nitrocellulose membrane embedded with recombinant antigens, it was sealed and stored at 2-8°C in the dark. |
| priorityDate | 2022-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.