http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114578041-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd5e90b280086281f802078453c85640 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5306 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 |
| filingDate | 2022-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5602251f3a7dd4b086a267ac050b238 |
| publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114578041-A |
| titleOfInvention | Preparation method and secondary antibody of polymer secondary antibody based on small molecule probe |
| abstract | The invention discloses a preparation method and a secondary antibody of a polymer secondary antibody based on a small molecule probe. In the method of the invention, a water-soluble polymer backbone is chemically modified, so that catalase is bound to the polymer through chemical bonds. The polymerase structure is formed on the surface, and after the small molecule probe with itaconic acid as the parent is combined with the antibody, the polymerase and the small molecule-antibody are mixed and incubated by the biochemical coupling method to form the polymerase-labeled. Secondary antibody. The above preparation method can prepare the polymer secondary antibody for the auxiliary diagnosis of solid tumor by immunohistochemistry. The parent of the probe is itaconic acid small molecule, which can realize the specific and efficient polymerization reaction of antibody and enzyme, so that more enzymes can be linked to the single-molecule antibody through the coupling structure to form a complex with good solubility and low steric hindrance structure, which can improve signal intensity in immunohistochemical experiments. The preparation method of the invention is safe and environmentally friendly, is easy to operate, does not need harsh conditions, and has high synthesis yield and purity. |
| priorityDate | 2022-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 217.