http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114574566-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44ae178fe5ea1fd193b8e5e1baf1ab3b |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02W10-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B50-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B40-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B40-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B50-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
filingDate | 2022-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa6e4c4514e26442f95923aae6d9e896 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c9f2e53328763740d34f4378d08758f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80df42516180696f1d1f4df33721febf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3b7f44025e0db6bca20e77049bb6b29 |
publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114574566-A |
titleOfInvention | Detection method and application of windmill grass on the diversity of constructed wetland matrix microorganisms |
abstract | The invention provides a method and application for detecting the influence of windmill grass on the diversity of constructed wetland matrix microorganisms, which comprises the following steps: (1) sample preparation and collection; (2) sample DNA extraction and detection of DNA concentration and concentration (3) PCR amplification; (4) high-throughput sequencing; (5) quality control, filtering, splicing and removal of chimeras; taxonomic analysis of OTU representative sequences, and then According to the OTU data, the alpha diversity is calculated to obtain the correlation index; by comparing the community composition difference of the matrix biofilm on the gravel surface under the condition of planting windmill grass, the present invention uses the high-throughput sequencing technology to amplify and sequence the bacterial 16S rDNA gene, and compare the results. For the diversity of microorganisms in different constructed wetland environments, it is possible to study their flora structure and dominant bacterial species, and provide help for the final design of a more scientific constructed wetland scheme. |
priorityDate | 2022-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.