http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114574553-A

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filingDate 2021-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41c3a8c1acdc1d78e0dabd7696ecb76d
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publicationDate 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114574553-A
titleOfInvention A kind of carbon dot-nano gold spherical nucleic acid and its preparation method and application
abstract The invention discloses a carbon dot-nano gold spherical nucleic acid and a preparation method and application thereof. The carbon dot-nano gold spherical nucleic acid takes nano gold as a core, and the surface is coupled with a DNAzyme chain and a substrate labeled with carbon dots at the end. When the carbon dot-nano gold spherical nucleic acid prepared by the present invention detects exosome miRNA, in the presence of metal ions, target miRNA recognition triggers DNAzyme walking to generate fluorescence; fluorescence spectrophotometer is used to measure the fluorescence signal of CDs after walking, Realize the detection and analysis of miRNA. At the same time, the present invention is based on two-color CDs-SNA for the detection of two exosome miRNAs, uses DNAzyme to mediate CDs fluorescence signal amplification, and realizes highly sensitive detection of miRNAs; uses two-color CDs-SNA to realize simultaneous detection of miRNAs, which is exosome The precise detection of miRNA provides a new method.
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