http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114574551-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2278c0815da18098af8cd142db85625 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2022-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3139bd99fbec732cf6567b6e92fd188 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d41d89e7d3f4b82fa514c9591ee55e2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69202778702fc7bbb61d78720a42910a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3eaf00a7a3b0c893899b9b94e4191ea8 |
| publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114574551-A |
| titleOfInvention | A method to detect off-target CRISPR gene editing |
| abstract | A method for detecting off-target CRISPR gene editing includes seven steps of cell cross-linking, antibody fixation, sonication, chromatin co-immunoprecipitation, DNA purification, DNA processing, and library amplification. Under the joint action of multiple steps, the present invention conducts immune co-settling of the protein-nucleic acid complex formed by Cas9 protein and target site and cell self-repair protein H2AX, MRE11 and the target site in the process of CRISPR/Cas9 gene editing, and Combining sequencing and other necessary technical means to screen off-target sites greatly simplifies the process of identifying off-target effects, while improving the accuracy of results, which can play a role in better predicting how genome editing will work in a clinical setting to strong technical support. It overcomes the limitations of the prior art, and has the disadvantages of long detection period and low sensitivity, as well as long detection process time, cumbersome steps, and inability to ensure that all breaking sites are marked. Based on the above, using the present invention has a good application prospect. |
| priorityDate | 2022-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.