http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114574425-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b6eefef0cc5a0e8088e08f39287984e8 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-092 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0627 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c8da8400810558aef30f8d5344d5c0a |
publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114574425-A |
titleOfInvention | A method for HHORSCs exosomes to promote dermal papilla cells to induce hair regeneration |
abstract | The present invention provides a method for HHORSCs exosomes to promote dermal papilla cells to induce hair regeneration, and the method for HHORSCs exosomes to promote dermal papilla cells to induce hair regeneration includes the following steps: S1, culturing and isolating from a human scalp sample Human dermal papilla cells and human hair follicle outer root sheath cells; S2. Evaluation of specific markers using flow cytometry and immunocytochemical techniques; S3. In platelets isolated from human hair follicle outer root sheath cells and human umbilical cord blood using ultracentrifugation Further isolation of exosomes; S4, detection of specific markers of exosomes by Western blotting; S5, analysis of particle size and distribution of exosomes by nanoparticle tracking analysis technology; S6, using proliferation assay, migration assay, etc. Methods to evaluate the effect of different concentrations of exosomes on regenerative capacity. The method for promoting HHORSCs exosomes to induce hair regeneration by dermal papilla cells provided by the present invention has the advantages of improving hair loss and realizing hair regeneration. |
priorityDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.