http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114563505-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b1b95a0151fe6cf57f119866e3a10c11 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-042 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2022-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73572a46949982d1758f52a23179a07b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcd69ced7f0125a7d7a000bc89d09996 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dad0a0bf738eaf7d206e1d83abb6047a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ade7f5b9bd0f91f552ed018ea0c50f9f |
| publicationDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114563505-A |
| titleOfInvention | Detection method of α-hydroxyceramide based on ultra-high performance liquid chromatography-mass spectrometry |
| abstract | The invention relates to the technical field of α-hydroxyceramide detection, in particular to a detection method of α-hydroxyceramide based on ultra-high performance liquid chromatography-mass spectrometry technology. Specifically, it includes the following steps: (1) mixing serum and extract, first standing, centrifuging to take the bottom layer to obtain material 1, mixing material 1 with the internal standard solution, volatilizing to dryness to obtain material 2, mixing material 2 with complex The solvent is mixed, the second standing is performed, and the supernatant is taken to obtain material 3; (2) the material 3 obtained in step (1) is subjected to ultra-high performance liquid chromatography tandem mass spectrometry analysis. Based on LC-MS/MS, the present invention establishes an analytical method for the simultaneous determination of six α-hydroxyceramides in serum for the first time, and the method has good precision, accuracy and wide linear range. |
| priorityDate | 2022-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.