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filingDate 2022-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3df6e93b0fb9d3dc0cdba7cd7b6bd75a
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publicationDate 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114563244-A
titleOfInvention A kind of lymphocyte chromosome karyotype preparation method
abstract The invention discloses a method for preparing lymphocyte chromosome karyotype, which comprises adding an anticoagulant to a freshly collected blood sample, preparing a single-cell suspension after inoculation and culturing, using a hypotonic solution for hypotonic treatment, and then fixing by karyotype. Liquid-fixed tablet, the anticoagulant sodium heparin and plasmin are composed, the hypotonic solution is a mixed solution of glycerol and KCl, and the karyotype fixative solution Carnot's fixative is added with dimethyl methacrylate base sulfoxide formation. The method improves the dispersion degree and stretchability of lymphocytes through the action of anticoagulant and hypotonic fluid, and the rapid fixation of the fixative improves the staining effect, and finally realizes the characteristics of clear and easy identification of stripes.
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