| abstract |
The invention belongs to the technical field of virus detection, and relates to a new coronavirus nucleic acid detection positive quality control product and a preparation method and use thereof. The PCR reaction solution is composed together, and the pseudovirus culture is obtained by constructing a recombinant plasmid from ORF1ab gene, N gene, E gene and tag gene, transposition, DNA separation and extraction, transfection, and separation. By detecting the tag gene, the present invention can timely and accurately distinguish the positive quality control substance from the novel coronavirus, so as to accurately determine whether it is laboratory contamination or novel coronavirus infection. |