| abstract |
The invention discloses the construction and application of a strain of Saccharomyces cerevisiae for extracellular transport of retinal and retinol, and belongs to the technical field of fermentation engineering. The invention constructs a recombinant Saccharomyces cerevisiae strain with enhanced retinal and retinol secretion capacity, and the recombinant Saccharomyces cerevisiae enhances the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase, isopentenyl Pyrophosphate isomerase, farnesyl pyrophosphate synthase, endoplasmic reticulum size regulator INO2 and ABC transporter Pdr10; heterologous expression of geranylgeranyl diphosphate synthase, phytoene dehydrogenase, 15-cis phytoene synthase, bifunctional phytoene cyclase/phytoene synthase, 15, 15'-dioxygenase; knockout of ROX1 and GAL80 genes in Saccharomyces cerevisiae; And down-regulated the expression of squalene synthase ERG9; when it was fermented in a shake flask, the extracellular retinal and retinol contents of the strain reached 43.50mg/L and 16.84mg/L, respectively. The total content is 73.40mg/L, so it has broad application prospects. |