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filingDate 2022-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85d32a4745b1cf43f55411629f83d614
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publicationDate 2022-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114544927-A
titleOfInvention A kind of method that utilizes graphdiyne oxide to detect mercury ion
abstract The invention belongs to the technical field of detection, and in particular relates to a method for detecting mercury ions by using graphdiyne oxide. In the invention, the DNA sequence with stable fluorescence is interacted with nanomaterial graphdiyne oxide (GDY), and the surface of GDY realizes the adsorption of single-stranded DNA through van der Waals force and π-π stacking between nucleic acid bases. Due to the principle of FRET, the fluorescence intensity changes; after Hg 2+ is added, Hg 2+ and the nitrogen atom at the third position of thymine in the DNA molecule undergo proton substitution, and the part of DNA with DSAI fluorescence is far away from the surface of GDY, nucleic acid aptamer The conformation of Hg 2+ changes to form a stable T‑Hg 2+ ‑T structural coordination complex, which is separated from the surface of GDY, and the fluorescence changes again; Hg 2+ is detected by the change of the absorbance of the system before and after adding Hg 2+ . The detection method provided by the invention is convenient to operate, low in cost, sensitive in detection and high in accuracy. The Hg 2+ detection applied in the fields of environment and food provides a new exploration direction for label-free fluorescent aptamer sensors.
priorityDate 2022-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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