http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114540292-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee4cab9f438045316adf7d596285fffa |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24152 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32452 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0668 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2022-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b00b79846d73aa2a9134b0c0a8f9f2ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3afc11b39e89f94321311dee189dd9d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39bbc6df948f4e125f1c9db24afc6ddc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44c474df0cab46c5d19a46b8d3914d35 |
| publicationDate | 2022-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114540292-A |
| titleOfInvention | A kind of suspension domestication method and application of duck embryo liver mesenchymal stem cells |
| abstract | The invention discloses a suspension domestication method of duck embryo liver mesenchymal stem cells. In the present invention, the isolated and cultured duck embryo liver mesenchymal stem cells are subjected to low-serum acclimation and serum-free suspension acclimation to obtain stably grown suspended serum-free duck liver mesenchymal stem cells. Through experiments, the inoculation process of different waterfowl viruses, such as inoculation ratio, culture time, and determination of harvesting standards, were determined to realize the adaptive growth of different viruses and increase the content of the corresponding viruses, laying the foundation for the research of waterfowl vaccines. The use of serum-free medium in the present invention greatly reduces the cost of cell culture, and reduces the probability of endogenous virus contamination due to the use of serum; the cells in suspension culture do not need trypsin digestion, the passage process is fast, and the damage to cells is less. , it is easier to expand the culture and harvest in batches; the virus can not only expand the culture by inoculating suspension cells, but also improve the titer of the virus to a certain extent. |
| priorityDate | 2022-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.