| abstract |
The invention discloses a chicken AQP1 mutant gene, a mutant protein, a gene mutant cell line and its application in cell suspension culture. The invention also discloses a preparation method of the AQP1 mutant cell line. By knocking out the spacer sequence after the first transmembrane region of the AQP1 protein of the chicken embryo fibroblast cell line DF-1 cell, the obtained AQP1 mutant cell line is easier to be acclimated in suspension, has stable hereditary traits, and has no tumorigenicity. The ability to proliferate the virus is not affected, and it can be applied to the production of poultry virus vaccines to replace the vaccine production process of solid-phase culture, which can greatly improve the production efficiency. |