| abstract |
The invention relates to a preparation method of a 3D printed tracheal stent loaded with stem cell exosomes, comprising the following steps: step (1), extraction and identification of bone marrow mesenchymal stem cell exosomes; step (2), 3D printing PCL tracheal stent Preparation; step (3), photocuring hydrogel loaded stem cell exosomes; step (4), preparation of composite tracheal scaffold loaded with stem cell exosomes; soaking the PCL scaffold printed in step (2) outside the loaded stem cells Exo-SilMA was soaked in the SilMA solution Exo-SilMA at 37°C for 30 minutes, and then cross-linked with a blue light for 5 minutes, so that Exo-SilMA evenly wrapped the PCL scaffold. Through the present invention, the PCL scaffold can provide good mechanical properties, while SilMA has good biocompatibility, biodegradability, and swelling effect, and can enrich biological components that promote tissue regeneration. Mesenchymal stem cell exosomes are used as stem cell derivatives , can exert the repair function of stem cells, and avoid the risks of tumorigenesis and rejection of transplanted stem cells. |