| abstract |
The invention provides a kit for inducing IPS cells into NK cells, comprising a first culture solution, a second culture solution, and a third culture solution; stem cell factors, human bone forming protein, vascular endodermis contained in the first culture solution A growth factor that can effectively induce the differentiation of mesoderm, Y‑27632 can significantly reduce the apoptosis of IPS cells. These components and other nutrients in the medium are used in the process of IPS cells to form Spin EB, which can promote the proliferation and differentiation of Spin EB. Stem cell factor, interleukin 7, interleukin 15 and tyrosine kinase receptor 3 ligands in the second and third medium, and interleukin 3 contained in the third medium can promote NK cells In the early stage of proliferation and differentiation, these components act synergistically in the various stages of Spin EB differentiation into NK cells, and can obtain high number and high purity NK cells. |