| abstract |
The invention provides a method for purifying high-purity tacrolimus. The operation method is as follows: the crude extract of tacrolimus produced by fermentation of Streptomyces tsukuba is used as a raw material, and the crude extract is treated with rare earth ions or silver ions to obtain a The high-purity fractions are collected, concentrated and crystallized to obtain pure tacrolimus. The method of the invention not only realizes the highly selective separation of tacrolimus and main impurities, but also effectively inhibits the isomer transformation. Simple and convenient, suitable for industrialization, large-scale production and other advantages. |