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publicationDate 2022-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114514311-A
titleOfInvention Methods for improving protein yield in fed-batch cell cultures
abstract In certain embodiments, the present disclosure provides a method of increasing the production of a recombinant polypeptide of interest, the method comprising: a) seeding mammalian cells in a feed fraction at a viable cell density of at least 5106 viable cells/ml in a batch bioreactor; and b) culturing the cells under optimized culture conditions to produce high titers of the recombinant polypeptide of interest.
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